fatemeh shahaliyan; Alireza Safahieh; Negin Salamat; Fatemeh Mojodi; Mostafa Zaredoost
Abstract
Due to their toxicities, oil pollutions and its derivatives such as anthracene are one of the most important issues of environmental researches all over the world. Oil hydrocarbons in contaminated soils, sediments and water can be degraded by microorganisms. Removal of hydrocarbons from aqueous solutions ...
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Due to their toxicities, oil pollutions and its derivatives such as anthracene are one of the most important issues of environmental researches all over the world. Oil hydrocarbons in contaminated soils, sediments and water can be degraded by microorganisms. Removal of hydrocarbons from aqueous solutions is also possible using cultured bacteria. In this study, the anthracene bioremediation by Bacillus pumilus isolated from oil sediments of Imam Khomeini port, was investigated. This bacterium made no clear halo on blood agar medium and couldn’t disperse the mineral oil but created a halo with a diameter of 0.5 cm on the surface of crude oil. As a result, the bacterium was detected as a non-producing biosurfactant species. The optical density of this species in the sample containing 30 mg/l of anthracene finally reached to 0.086. Measurement of anthracene degradation by HPLC showed that %40.833 of mentioned hydrocarbon was removed after 5 days. Therefore, the results indicate that the isolated bacterium can probably behave the same in the natural environment and could have the potential in bioremediation of this pollutant from environments.
Rahman Alimi; Ahmad savari; Abdolali Movahedinia; Mohammad Zakeri; Negin salamat
Abstract
The Brown banded Bamboo shark is a benthic shark and unlike most of shark, to attract the oxygen in the water no need to move constantly. In this investigation, 36 individual of Brown banded Bamboo sharks (Chiloscyllium punctatum) were caught from Dervish's Creek in the northern Persian Gulf. Certain ...
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The Brown banded Bamboo shark is a benthic shark and unlike most of shark, to attract the oxygen in the water no need to move constantly. In this investigation, 36 individual of Brown banded Bamboo sharks (Chiloscyllium punctatum) were caught from Dervish's Creek in the northern Persian Gulf. Certain sections of the gill tissue removed and immediately fixed in Bouin’s solution. Then embedded the samples in paraffin and sectioned into 5 micrometer microscopic and stained with hematoxylin and eosin. In order to evaluate the comprehensive, sagittal and cross-section of gill tissue were studied. Microscopic results of cross-section indicated that the gill tissue structures including, filaments and lamella sections revealed. In addition, a variety of cells such as columnar cells and chloride cells (MR) were also detected. According to the results obtained for the cross section, The cell aggregates, named follicularlyarranged NKA-rich cells (follicular NRCs), were found in the gills septum. Therefore, the Brown banded Bamboo shark has three types of NKA-rich cells in the gill: (1) NKA-rich MR cells on the filament and lamella, (2) NKA-rich solitary cells (solitary NRCs) and (3) follicular NRCs on the gill septum.
علوم زیستی دریا
Rahman Alimi; Ahmad Savari; Abdolali Movahedinia; Mohammad Zakeri; Negin Salamat
Abstract
Cartilaginous fishes were used urea for osmoregulation of their internal fluids. There are several studies on osmoregulation of sharks but investigate the role of reproduction season and body lenght on the concentration of osmolytes is necessary. In this investigation, 36 individual of Brown banded Bamboo ...
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Cartilaginous fishes were used urea for osmoregulation of their internal fluids. There are several studies on osmoregulation of sharks but investigate the role of reproduction season and body lenght on the concentration of osmolytes is necessary. In this investigation, 36 individual of Brown banded Bamboo sharks (Chiloscyllium punctatum) in both autumn (before reproduction) and spring (period of reproduction) seasons were caught from Moussa Creek located at the northern of Persian Gulf. After weighing, biometry, sex determination and the numbering of sharks. Electrolyte analyzer and auto analyzer were used to measuring electrolyte and urea concentration. The results showed that blood osmolytes of sharks in the male and female sex were no significant differences (P>0/05). But, there was a significant difference between the concentrations of these substances in the autumn and spring season (P
Abstract
Among more than 600 fish species in Persian Gulf, Soles are considered as one of the most economically important species. These fish is different from other fishes in morphological , functional and structural organs and like many other flatfish are less well known. The present study was designed to investigate ...
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Among more than 600 fish species in Persian Gulf, Soles are considered as one of the most economically important species. These fish is different from other fishes in morphological , functional and structural organs and like many other flatfish are less well known. The present study was designed to investigate the histological structure of gonads of Sole (Euryglossa orientalis) in two breeding and rest seasons. 40 Sole fish were collected from Zangi estuary (located in Mussa creek in the north of Persian Gulf) in early July and early October(non- breeding and breeding seasons) 2013. After biometry, gonadal tissues were dissected and weighed to determine Gonadosomatic Index (GSI). Then fixed in Bouin 's solution. Histological specimens were transported to the histological laboratory. Tissue sections were prepared according to conventional methods include dehydration, clearing and paraffin. And then were cut down to 5 micron sections and stained. These tissue sections were studied by light microscopy . The result showed that different stages of germ cells in both male and female gonads. Although in breeding season mature cells were noticed, GSI index of testis and ovary in Sole showed significant difference between the two seasons.
maryam khaniyan; negin salamat; alireza safahieh; abdolali movahedinia
Abstract
Benzo[a]pyrene (BaP), is a ubiquitous environmentalcontaminant present in air, water, andsediment . BaP is readily absorbed by all routes of exposure and can pass directly through the plasma membrane of most cell types. The present study was conducted to examine the effects of BaPon liver tissue structure ...
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Benzo[a]pyrene (BaP), is a ubiquitous environmentalcontaminant present in air, water, andsediment . BaP is readily absorbed by all routes of exposure and can pass directly through the plasma membrane of most cell types. The present study was conducted to examine the effects of BaPon liver tissue structure in orange spotted grouper Epinepheluscoioidesaffected by different concentration of BaP.In the present report, 100 E.coioides were exposed by intraperitoneal injection to 2, 20 and 200 mg/kg of BaPfor 14d under laboratory conditions and the samples were taken from fish liver in days 0, 2, 4, 7 and 14 of experiment for histopathological study. The liver sampleswere fixed in 10%formalin buffer solution. Five-micrometer thick sections were obtained and were stained in hematoxylin/eosin for examination in light microscopy.No pathological alteration was observed in the liver samples of controls fish. The pathological alterations of liver were including of hepatocyte vacuoletion, increase of melanomacrophagecenters, dilation of dissspace, sinusoidal dilation, focal necrosis, hepatocytes hypertrophy, nucleus in a lateral positionand hepatocyte degeneration. The frequency and severity of tissue changes raised with the increase of BaP concentration in all sampling days. The most frequency and severity of histopathological alteration were observed in fish treated by 200 mg/kg BaP in day 7.